PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:2000 | Species independent |
| ICC | 1:500 | Species independent |
Cas9, derived from the bacteria Streptococcus pyogenes, is a key component of the CRISPR-Cas9 gene-editing system. It is an RNA-guided DNA endonuclease that can be programmed to target and cut specific DNA sequences within the genome. The Cas9 protein, along with a single-guide RNA (sgRNA), forms a complex that recognizes a specific sequence of DNA preceding a short sequence known as a PAM (protospacer adjacent motif) and introduces a double-strand break at that location. This ability allows for precise gene editing through mechanisms such as non-homologous end joining (NHEJ) or homology-directed repair (HDR), enabling researchers to knockout genes, insert new genetic material, or make specific changes to the DNA sequence. Cas9's high specificity and programmability have made it a powerful tool in fields ranging from basic research to potential therapeutic applications in treating genetic disorders and cancer. Recent advancements have also focused on improving the precision and reducing off-target effects of Cas9, making it a safer and more efficient tool for gene editing.
WB result of Cas9 Recombinant Mouse mAb
Primary antibody: Cas9 Recombinant Mouse mAb at 1/2000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 5 µg
Lane 2: 293T transfected with Cas9-Myc-His fusion protein whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 170 kDa
ICC shows positive staining in Cas9-Myc-His transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- Cas9 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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