12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICFCM | 1:2000 | Hu |
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte), treated overnight with 500ng/ml LPS (Red) or untreated (Green), labeling IL-6 at 1/2000 dilution (0.1 μg) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
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