PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
MCP-1, also known as CCL2, is a chemokine that plays a central role in inflammation and immune responses. It is primarily produced by various cell types, including monocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells. MCP-1 is crucial for the recruitment, activation, and migration of leukocytes, particularly monocytes and macrophages, to the site of inflammation. It is involved in the pathogenesis of numerous diseases, including COVID-19, cancers, neuroinflammatory diseases, rheumatoid arthritis, and cardiovascular diseases, by promoting the migration and infiltration of inflammatory cells. Additionally, MCP-1 contributes to insulin resistance, diabetes, and its complications, such as diabetic nephropathy and retinopathy, by increasing the level of circulating MCP-1 in type 1 and type 2 diabetes. Furthermore, it plays a role in cancer by recruiting macrophages to the tumor microenvironment, promoting angiogenesis, and inducing cell invasion and metastasis. Overall, MCP-1 is a key chemoattractant with functions that extend beyond immunity, influencing a wide range of pathological processes.
WB result of MCP1 Recombinant Rabbit mAb
Primary antibody: MCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha for 6 hours and 1 µg/ml Brefeldin A for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 11~13 kDa
ICC analysis of HeLa cells treated with TNF-alpha(20 ng/ml, 6 hr)add Brefeldin A (1 µg/m, 3 hr) (top panel) and untreated HeLa cells (below panel). Anti- MCP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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