12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
P4HB protein, also known as prolyl 4-hydroxylase subunit beta, is a multifunctional protein that belongs to the protein disulfide isomerase family. It serves as an oxidoreductase, isomerase, and molecular chaperone, and is involved in the hydroxylation of prolyl residues in preprocollagen, the formation, breakage, and rearrangement of disulfide bonds, and the inhibition of aggregation of misfolded proteins. P4HB is found in various cellular compartments, including the endoplasmic reticulum, mitochondria, cytosol, and the cell surface. It also plays a role in the regulation of reactive oxygen species (ROS) generation, prevention of ER stress, and protein misfolding. Additionally, P4HB has been implicated in several types of cancer and may be associated with immune responses, as it strongly co-expresses with immune-related genes and is involved in antigen processing and presentation, as well as protein processing in the endoplasmic reticulum. High expression levels of P4HB are correlated with worse prognosis in various cancers, suggesting its potential as a diagnostic biomarker and therapeutic target.
WB result of P4HB Recombinant Rabbit mAb
Primary antibody: P4HB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 60 kDa
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-P4HB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-P4HB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-P4HB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-P4HB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-P4HB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in A431 cells. Anti- P4HB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of P4HB in tumor tissue.
Expression of P4HB in human tissue.
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