PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Species Independent |
| FCM | 1:800 | Species independent |
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
Myc-His tag transfected 293T (Human embryonic kidney epithelial cell (Right panel) or 293T (Left panel) was stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (Black line histogram) and SDT His tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
ICC shows positive staining in Histone H3-myc-his Tag transfected 293T cells. Anti-His tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows negative staining in vector-transfected 293T cells. Anti-His tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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