| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Ms |
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen-presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Flow cytometric analysis of Mouse CD8α expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 antibody and either Phycoerythrin Rat IgG1, λ Isotype Control (Left panel) or SDT PE Rat Anti-Mouse CD8α Antibody (Right panel) at 0.1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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