Cell culture supernatant, Serum, Plasm, Cell/Tissue Lysate
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. The interleukin-1 system (IL-1) is a prominent pro-inflammatory pathway responsible for the initiation and regulation of immune responses, but it has also received much attention for its pleiotropic neuromodulator effects under physiological and pathophysiological conditions. In particular, its main cytokine ligand, IL-1β, has emerged as a key regulator of the ethanol-induced neuroimmune response, contributing to ethanol drinking and the development of ethanol dependence. Human genetic studies have found polymorphisms in genes encoding components of the IL-1β signaling pathway and IL-1β associated with increased susceptibility to alcoholism, and IL-1β levels are elevated in the periphery and brain of alcoholic patients. Therefore, elucidating the mechanistic role of IL-1β in ethanol drinking and dependence is critical for understanding disease progression, as well as for the identification of novel therapeutic targets.
Standard curve
Example of Mouse IL-1β standard curve in Assay Diluent #1.
Linearity
The concentrations of IL-1β were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 5ug/ml LPS and 100ng/mL rmIL-10 for 7days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 47.62 pg/mL in stimulated RAW264.7 supernatant.
Linearity
#2 sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS for 48 hours (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 83.39 pg/mL in stimulated RAW264.7 supernatant.
#3 sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 100 ng/ml LPS for 4 hours and 1000 ng/ml Brefeldin A for 3 h. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6040.05 pg/mL in stimulated RAW264.7 lysate (4mg/mL).
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