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Argonaute-2 Recombinant Rabbit mAb (S-1826-47)

Argonaute-2 Recombinant Rabbit mAb (S-1826-47)

货号: S0B1246
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Argonaute-2

  • 分子别名

    Protein argonaute-2; hAgo2; Argonaute2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C (eIF-2C; eIF2C 2); PAZ Piwi domain protein (PPD); Protein slicer; AGO2;

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q9UKV8

  • 克隆号

    S-1826-47

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, Jurkat, MCF7, HepG2, U-87 MG, RAW264.7, C6

  • 预测反应种属
    (反应种属缩写表)

    Bv, Rb, Xe,

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:2000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu
背景介绍

  • Argonaute-2 (AGO2) is a pivotal protein in the RNA interference and microRNA pathways, known for its role in gene regulation through the binding and cleavage of target mRNAs, which it achieves by forming the RNA-induced silencing complex (RISC) with small RNAs such as siRNA and miRNA. This process is essential for post-transcriptional gene regulation, immune defense, and plays a significant role in various biological processes including development, cell differentiation, and tumorigenesis.

  • 免疫印迹

    • WB result of Argonaute-2 Recombinant Rabbit mAb
      Primary antibody: Argonaute-2 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: U-87 MG whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 100 kDa

    • WB result of Argonaute-2 Recombinant Rabbit mAb
      Primary antibody: Argonaute-2 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 90 kDa

    • WB result of Argonaute-2 Recombinant Rabbit mAb
      Primary antibody: Argonaute-2 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 90 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-Argonaute-2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Argonaute-2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Argonaute-2 in tumor tissue.

    • Expression of Argonaute-2 in human tissue.

    • Expression of Argonaute-2 in mouse & rat tissue.