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EHD2 Recombinant Rabbit mAb (S-1580-60)

EHD2 Recombinant Rabbit mAb (S-1580-60)

货号: S0B1247
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    EHD2
  • 分子别名

    EH domain-containing protein 2; PAST homolog 2; PAST2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Cell membrane
  • Accession

    Q9NZN4
  • 克隆号

    S-1580-60
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HeLa, A549, HCT 116
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu
IHC-P 1:1000 Hu, Ms, Rt
背景介绍
  • EHD2, a member of the EH domain-containing protein family, is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. It functions as a nuclear-cytoplasmic shuttle protein and acts as a corepressor of transcription within the nucleus to inhibit gene transcription. EHD2 plays a key role in endocytic transport, being involved in the formation and maintenance of endosomal tubules and vesicles, which are essential for intracellular transport of proteins and other substances. The N-terminus of EHD2 is attached to the cell membrane, while its C-terminus binds to actin-binding proteins, linking the actin cytoskeleton to membrane curvature and promoting cell endocytosis. Additionally, EHD2 is associated with membrane protein trafficking, receptor signaling, glucose metabolism, and lipid metabolism, highlighting its multifaceted role in cellular processes and its potential implications in diseases such as cancer and metabolic disorders.

  • 免疫印迹

    • WB result of EHD2 Recombinant Rabbit mAb
      Primary antibody: EHD2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: SW620 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: A549 whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Negative control: SW620 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 61 kDa
      Observed MW: 68 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of EHD2 in tumor tissue.

    • Expression of EHD2 in human tissue.