12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu, Ms, Rt |
IFIT1 (Interferon-induced protein with tetratricopeptide repeats 1) is a member of the ISG (Interferon-stimulated genes) family, which is induced upon interferon treatment or viral infection. Primarily located in the cytoplasm, IFIT1 contains multiple tetratricopeptide repeat (TPR) motifs that facilitate protein-protein interactions. It inhibits viral infection by recognizing and binding to viral RNAs that lack the 2'-O-methylated cap structure or possess a 5'-triphosphate group, thereby suppressing viral translation or sequestering them. Additionally, IFIT1 interacts with the eIF3e subunit of the eukaryotic translation initiation factor 3, inhibiting translation, and has been shown to suppress the replication of various RNA viruses.
WB result of IFIT1 Recombinant Rabbit mAb
Primary antibody: IFIT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml hIFN-α for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of IFIT1 in tumor tissue.
Expression of IFIT1 in human tissue.
Expression of IFIT1 in mouse & rat tissue.
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