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α-tubulin (acetyl K40) Recombinant Rabbit mAb (S-1536-100)

α-tubulin (acetyl K40) Recombinant Rabbit mAb (S-1536-100)

货号: S0B1236
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    α-tubulin (acetyl K40)
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoskeleton
  • Accession

    P68366
  • 克隆号

    S-1536-100
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 预测反应种属
    (反应种属缩写表)

    Bv, Ck, Cz, Hm, Pg, Xe
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
背景介绍
  • α-Tubulin acetylation at lysine 40 (α-tubulin K40ac) is a post-translational modification that marks long-lived microtubules and plays a role in various cellular functions. This acetylation is associated with the stabilization of microtubules, particularly in structures like axons and cilia. It is believed to influence microtubule dynamics, potentially by affecting lateral interactions among protofilaments and increasing microtubule stability. α-Tubulin K40 acetylation is also implicated in intracellular transport, cell division, and cell migration, as well as in neuronal development, including axonal transport, neuronal migration, and axonal growth. Furthermore, it has been suggested that acetylated microtubules facilitate the transport of the Hippo regulator Merlin, which is crucial for contact inhibition of proliferation and cell-substrate adhesion. The modification is regulated by the acetyltransferase αTAT1 and deacetylases like HDAC6 and SIRT2, and it serves as a stable microtubule marker, enhancing the binding of motor proteins and playing a role in early polarization events in neurons. Deficiency in α-tubulin acetylation has been linked to impaired axonal transport and neurodegenerative disorders. Thus, α-tubulin (acetyl K40) is critical for maintaining the structural integrity of microtubules and regulating key cellular processes.

  • 免疫印迹

    • WB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
      Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 400 nM TSA for 16 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
      Observed MW: 55 kDa

    • WB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
      Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
      Observed MW: 55 kDa

    • WB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
      Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
      Observed MW: 55 kDa

  • 斑点杂交

    • Dot blot result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
      Lane 1: α-tubulin K40 acetyl peptide
      Lane 2: α-tubulin K40 acetyl peptide
      Lane 3: α-tubulin K40 unmodified peptide
      Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression ofα-tubulin (acetyl K40) in tumor tissue.

    • Expression ofα-tubulin (acetyl K40) in human tissue.

    • Expression ofα-tubulin (acetyl K40) in mouse & rat tissue.