PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu,Ms,Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu,Ms,Rt |
LC3A and LC3B are members of the LC3/GABARAP protein family, playing crucial roles in the process of autophagy. These proteins are involved in the formation and maturation of autophagosomes, which are essential for the cell's degradation and recycling of damaged or unnecessary components. During autophagy, LC3A and LC3B are cleaved from their precursor proteins (Pro-LC3) to form LC3-I, which then get converted to LC3-II by binding to phosphatidylethanolamine (PE), signifying the formation of autophagosomes. LC3-II is a component of the autophagosome membrane, and its accumulation is commonly used as a biomarker for autophagy activity. While LC3A and LC3B are functionally similar, their expression may vary across different cell types and tissues, suggesting they might have specific regulatory roles in the autophagy process. In summary, the functions of LC3A and LC3B are primarily associated with the formation of autophagosomes and the regulation of autophagic flux, which are vital for maintaining cellular homeostasis and responding to nutrient deprivation.
WB result of LC3A/B Recombinant Rabbit mAb
Primary antibody: LC3A/B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 μM Chloroquine overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 14 kDa
Observed MW: 14, 16 kDa
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-LC3A/B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-LC3A/B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-LC3A/B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC analysis of HeLa cells treated with Chloroquine (50μM,24h) (top panel) and HeLa cells untreated with Chloroquine (50μM,24h) (below panel). Anti-LC3A/B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of LC3A/B in tumor tissue.
Expression of LC3A/B in human tissue.
Expression of LC3A/B in mouse & rat tissue.
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