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Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb (S-1127-19)

Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb (S-1127-19)

货号: S0B1233
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-NF-kB p105/p50 (Ser337)

  • 分子别名

    DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P19838

  • 克隆号

    S-1127-19

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Ck, Dg

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:5000 Hu, Ms, Rt
背景介绍

  • Phospho-NF-kB p105/p50 (Ser337) is a phosphorylated form of the NF-kB subunits p105 and p50, which are crucial in the regulation of gene expression, particularly in response to various stimuli such as inflammation, immunity, differentiation, cell growth, and tumorigenesis. Phosphorylation at Ser337 is significant for the transcriptional activity of these subunits. It has been shown that phosphorylation at this site can affect the dimerization of p50 and its interaction with DNA. Specifically, phosphorylation by protein kinases like PKAc and Chk1 influences the ability of p50 to form homodimers and bind to DNA, which in turn modulates the transcriptional output of genes under the control of NF-kB. This phosphorylation event is also implicated in the gene-specific regulation of transcription, offering opportunities for selective targeting of NF-kB for therapeutic benefits. Thus, Phospho-NF-kB p105/p50 (Ser337) is a critical regulatory point in the NF-kB signaling pathway, impacting the transcriptional program that governs key physiological processes and disease states.

  • 免疫印迹

    • WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
      Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
      Observed MW: 50, 110 kDa

    • WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
      Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 20 ng/ml TNF-alpha and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
      Observed MW: 55 kDa

    • WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
      Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
      Observed MW: 60, 110 kDa

  • 斑点杂交

    • Dot blot result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
      Lane 1: NF-kB p105/p50 (Ser337) phospho peptide
      Lane 2: NF-kB p105/p50 unmodified peptide
      Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution