12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
ATPB refers to the "ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide," which is a subunit of the mitochondrial ATP synthase enzyme. This enzyme is crucial for cellular energy production as it catalyzes the synthesis of ATP, utilizing the electrochemical gradient of protons across the inner mitochondrial membrane during oxidative phosphorylation. ATPB is one of the subunits that make up the F1 complex, the soluble catalytic core of ATP synthase, which is composed of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other three. The protein is also known by several synonyms including ATP5B, ATPMB, ATPSB, and is associated with the gene symbol ATPB. The molecular weight of the protein is approximately 57 kDa, with a calculated molecular weight of 529 amino acids. ATPB plays a significant role in the process of ATP synthesis within the mitochondria, highlighting its importance in cellular metabolism and energy production.
WB result of ATPB Rabbit pAb
Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Lane 5: HL-60 whole cell lysate 20 µg
Lane 6: LoVo whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 50 kDa
WB result of ATPB Rabbit pAb
Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 50 kDa
WB result of ATPB Rabbit pAb
Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 50 kDa
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung carcinoma. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of ATPB in tumor tissue.
Expression of ATPB in human tissue.
Expression of ATPB in mouse & rat tissue.
您现在的位置：