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Rat IgM OneStep ELISA Kit

Rat IgM OneStep ELISA Kit

货号: S0C3027
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    IgM
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Rt
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 4.4%;

      Inter-assay: 5.1%

    • Sample type

      Cell culture supernatant, Serum, Plasm
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      1.818 ng/ml
    • Range

      15.63ng/mL – 1000 ng/mL
    • Recovery

      Cell culture supernatant 97%
      Serum 93%
      Plasm 99%
    • Assay time

      60 minutes
    • Species reactivity

      Rat
背景介绍
  • Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. IgM is the first immunoglobulin expressed in the human fetus (around 20 weeks) and phylogenetically the earliest antibody to develop. IgM antibodies do not pass across the human placenta (only isotype IgG). Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection (e.g. congenital rubella syndrome). IgM in normal serum is often found to bind to specific antigens, even in the absence of prior immunization. A mutation of the mu chain within IgM causes autosomal recessive agammaglobulinemia. The presence of IgM or, rarely, IgG is one of the obligate criteria for a diagnosis of Schnitzler's syndrome.

  • ELISA

    • Standard curve
      Example of Rat IgM standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Rat serum was 2×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 377.84 ug/mL in Rat serum.

    • Linearity
      #2 sample is undiluted samples are as follows: Rat plasma was 2×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 202.15 ug/mL in Rat plasma.

    • Protocol Diagram