12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
Rabbit Anti-V5 Agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant V5 tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant V5 tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-V5 Agarose Beads. It is the excellent choice for high performance purifications.
Rabbit Anti-V5 Agarose Beads is used to immunoprecipitate V5-tagged fusion proteins from Escherichia coli lysate supernatant. During elution with Peptide elution buffer, the enriched V5-tagged fusion proteins are released from Rabbit Anti-V5 Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: Flow-through
Lane 4: V5-tagged fusion protein
Lane 5: V5-tagged fusion protein