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IRE1α Recombinant Rabbit mAb (S-1356-124)

IRE1α Recombinant Rabbit mAb (S-1356-124)

货号: S0B1208
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    IRE1α
  • 分子别名

    Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1 (hIRE1p); Ire1-alpha (IRE1a); ERN1; IRE1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Endoplasmic reticulum membrane
  • Accession

    O75460
  • 克隆号

    S-1356-124
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    A549, HCT 116, 293T, MDA-MB-231, U-2 OS, SK-BR-3, Neuro-2a, C2C12
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms
背景介绍
  • IRE1α is a type I transmembrane protein that senses misfolded proteins in the ER. Upon activation, it undergoes dimerization and autophosphorylation, which activates its endoribonuclease (RNase) activity. This leads to the splicing of X-box binding protein 1 (XBP1) mRNA, producing the active transcription factor XBP1s, which regulates the expression of genes involved in alleviating ER stress. In addition to splicing XBP1, IRE1α also mediates a process known as regulated IRE1-dependent decay (RIDD), which selectively degrades certain mRNAs to reduce the load of new proteins entering the ER, thereby helping to restore ER homeostasis. IRE1α has been implicated in various cancers, including triple-negative breast cancer (TNBC). Its activation in cancer cells can promote tumor progression by fostering an immunosuppressive tumor microenvironment. Targeting IRE1α has shown potential in enhancing anti-tumor immunity and the effectiveness of immunotherapies. Studies indicate that inhibiting IRE1α can lead to significant tumor regression and improved survival rates in mouse models of cancer. Specifically, IRE1α loss has been associated with reduced immunosuppressive cells and increased CD8+ T cell populations in the tumor microenvironment.

  • 免疫印迹

    • WB result of IRE1α Recombinant Rabbit mAb
      Primary antibody: IRE1α Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: HCT 116 whole cell lysate 20 µg
      Lane 3: 293T whole cell lysate 20 µg
      Lane 4: MDA-MB-231 whole cell lysate 20 µg
      Lane 5: U-2 OS whole cell lysate 20 µg
      Lane 6: SK-BR-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 110 kDa
      Observed MW: 115 kDa

    • WB result of IRE1α Recombinant Rabbit mAb
      Primary antibody: IRE1α Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 110 kDa
      Observed MW: 115 kDa