12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phosphorylation of NF-κB p65 at Ser529 is a post-translational modification that plays a significant role in the regulation of NF-κB activity. This modification is catalyzed by casein kinase II (CK2) upon stimulation by cytokines such as interleukin-1β (IL-1β) or tumor necrosis factor α (TNFα), leading to increased p65 transactivation potential in a gene-specific manner. Phosphorylation at S529 enhances the transcriptional activity of NF-κB, which is crucial for regulating various cellular processes including inflammation, immune responses, and cell survival. Dysregulation of NF-κB signaling, including phosphorylation at S529, has been implicated in various diseases, particularly in the context of chronic inflammation and aging. Phosphorylation of p65 at S529 has also been reported to promote tumorigenesis in various tissues, including the bone, breast, and head and neck, suggesting a significant role of NF-κB phosphorylation in different types of cancers.
WB result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha for 5 miniutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
Observed MW: 65 kDa
Dot blot result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
Lane 1: NF-κB p65 (S529) phospho peptide
Lane 2: NF-κB p65 unmodified peptide
Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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