12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000-1:10000 | Hu, Ms |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu, Ms |
S100A4 is a member of the S100 protein family and is a calcium-binding protein with an EF double helix domain. It promotes the metabolic reprogramming of M2-type tumor-associated macrophages (TAMs) by enhancing the utilization of extracellular fatty acids and increasing mitochondrial-dependent fatty acid oxidation (FAO) levels. This controls the transformation of M2-type TAMs towards a "pro-tumor" phenotype. Deletion of S100A4 in mice leads to a significant reduction in the proportion of M2-type TAMs in the tumor microenvironment (TME) and inhibits tumor growth. S100A4 is significantly upregulated in various inflammatory cells such as T lymphocytes, dendritic cells, and macrophages in the dermis of psoriatic skin compared to healthy skin, indicating its role as an active inflammatory cytokine in psoriasis pathogenesis.
WB result of S100A4 Recombinant Rabbit mAb
Primary antibody: S100A4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A375 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 12 kDa
Observed MW: 12 kDa
WB result of S100A4 Recombinant Rabbit mAb
Primary antibody: S100A4 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 12 kDa
Observed MW: 12 kDa
IHC shows positive staining in paraffin-embedded human colon. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-S100A4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in A375 cells. Anti- S100A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- S100A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of S100A4 in tumor tissue.
Expression of S100A4 in human tissue.
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