12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms |
| FCM | 1:2000 | Ms |
Mouse I-A/I-E refers to the murine major histocompatibility complex class II (MHC class II) molecules, which play a central role in the immune system by presenting antigens to T cells. The I-A and I-E molecules are expressed on the surface of antigen-presenting cells (APCs), such as B cells, macrophages, and dendritic cells, and are responsible for displaying peptides derived from extracellular proteins to CD4+ T cells. This interaction is crucial for initiating the adaptive immune response. Antibodies against mouse I-A/I-E can be used in various scientific applications, including flow cytometry, immunohistochemistry, and immunoprecipitation. These antibodies can target MHC class II molecules in different species, including mice, and are essential tools for research in immunology and cell biology.
WB result of Rat Anti-Mouse I-A/I-E Antibody
Primary antibody: Rat Anti-Mouse I-A/I-E Antibody at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25~35 kDa
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse I-A/I-E antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Rat IgG2a, κ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Alexa Fluor® 647 antibody separately.
您现在的位置：