12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:10000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Peroxiredoxin 1 (PRDX1) is a member of the peroxiredoxin family of antioxidant enzymes, which are ubiquitously distributed in all species and capable of reducing a broad range of peroxide substrates, including hydrogen peroxide (H2O2) and alkyl hydroperoxides. PRDX1 plays a crucial role in cellular redox homeostasis and is involved in the regulation of various cellular processes such as proliferation, differentiation, and apoptosis. PRDX1 has been implicated in the development and progression of various human cancers, including breast, esophageal, lung, and prostate cancers, where it regulates ROS-dependent signaling pathways and is thought to be a key intracellular intermediate balancing cell survival and apoptosis.
WB result of Peroxiredoxin 1/PAG Recombinant Rabbit mAb
Primary antibody: Peroxiredoxin 1/PAG Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 22 kDa
WB result of Peroxiredoxin 1/PAG Recombinant Rabbit mAb
Primary antibody: Peroxiredoxin 1/PAG Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 22 kDa
IHC shows positive staining in paraffin-embedded human liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti- Peroxiredoxin 1/PAG antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of Peroxiredoxin 1/PAG in tumor tissue.
Expression of Peroxiredoxin 1/PAG in human tissue.
Expression of Peroxiredoxin 1/PAG in mouse & rat tissue.
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