应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 0.1μg per million cells in 100μl volume | Hu |
CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).
Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with SDT Biotin Mouse Anti- Human CD14 Antibody at 0.1 μg/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.