Intra-assay: 3.2%；
Inter-assay: 6.8%
Mouse immunoglobulin M (IgM) is a crucial component of the humoral immune system and is the first antibody produced by B-cells in response to an antigenic challenge. It exists in two forms: a pentameric (19S) form found in serum and a monomeric (8S) form on the surface of lymphocytes. The IgM pentamer, which is the predominant form in circulation, consists of five monomeric units and has a molecular weight of approximately 900 kDa, while the hexameric form has a molecular weight of about 1050 kDa. Each monomer of IgM is composed of two heavy chains and two light chains, with the heavy chain having four constant domains (CH1-CH4) and the light chain having one constant domain (CL). The CH4 domain facilitates oligomerization, leading to the formation of penta- or hexamers, and these oligomers are covalently linked via disulfide bonds, resembling a snowflake structure. A joining (J) chain, a 15 kDa polypeptide, is essential for the secretion of IgM into mucosal areas.
Standard curve
Example of Mouse IgM standard curve in Assay Diluent #1.
Linearity
The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse serum was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 262.88 ug/mL in Mouse serum.
#2 sample is undiluted samples are as follows: Mouse plasma was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 335.85 ug/mL in Mouse plasma.
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