PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IF | 1:100 | Hu |
CD45RO is an important cell surface marker in immunology that belongs to the CD45 family (also known as Leukocyte Common Antigen, LCA). CD45 is a highly polymorphic transmembrane protein primarily expressed on the surface of white blood cells, including T cells, B cells, natural killer (NK) cells, monocytes, and granulocytes, and plays a crucial role in lymphocyte development, activation, and signaling. Specifically, CD45RO is an isoform of CD45 that is highly expressed on memory T cells but expressed at lower levels or not expressed on naive T cells. Memory T cells are T cells that have been activated and survived during previous immune responses, enabling them to rapidly and strongly activate upon re-encountering the same antigen, thus accelerating and enhancing immune responses. Therefore, CD45RO is often used to distinguish and identify memory T cells. It serves as an important marker in immune phenotyping analysis, disease diagnosis, immune response monitoring, and immunotherapy research.
IF shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RO (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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