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Sarcomeric Alpha Actinin Recombinant Rabbit mAb (S-1652-40)

Sarcomeric Alpha Actinin Recombinant Rabbit mAb (S-1652-40)

货号: S0B1149
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Sarcomeric Alpha Actinin
  • 分子别名

    Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm
  • Accession

    P35609
  • 克隆号

    S-1652-40
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB, IF
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    C2C12, mouse heart, rat heart, rat skeletal muscle
  • 预测反应种属
    (反应种属缩写表)

    Bv, Ck, Mq
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
IF 1:500 Ms
背景介绍
  • Sarcomeric Alpha Actinin belongs to the spectrin superfamily, which includes spectrin, dystrophin, and their homologues and isoforms. It forms an anti-parallel rod-shaped dimer with one actin-binding domain at each end, bundling actin filaments in various cell types and cytoskeleton frameworks. In striated, cardiac, and smooth muscle cells, Sarcomeric Alpha Actinin is localized at the Z-disk and analogous dense bodies, where it forms a lattice-like structure and stabilizes the muscle contractile apparatus. Alpha Actinin functions as an actin crosslinker in muscle cells, anchoring actin filaments at the Z lines in striated muscles and dense bodies in smooth muscle cells. It plays a role in muscle contraction by interacting with the contractile machinery. In non-muscle cells, Sarcomeric Alpha Actinin is found along actin filaments and in adhesion sites, where it contributes to the organization of the cytoskeleton and cell adhesion. Sarcomeric Alpha Actinin is also implicated in autoimmune diseases, particularly systemic lupus erythematosus and autoimmune hepatitis, where it may serve as a target autoantigen.

  • 免疫印迹

    • WB result of Sarcomeric Alpha Actinin Recombinant Rabbit mAb
      Primary antibody: Sarcomeric Alpha Actinin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C2C12 whole cell lysate 20 µg
      Lane 2: mouse heart lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 104 kDa
      Observed MW: 110 kDa

    • WB result of Sarcomeric Alpha Actinin Recombinant Rabbit mAb
      Primary antibody: Sarcomeric Alpha Actinin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Lane 2: rat skeletal muscle lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 104 kDa
      Observed MW: 110 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human skeletal cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Sarcomeric Alpha Actinin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse heart. Anti-Sarcomeric Alpha Actinin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of Sarcomeric Alpha Actinin in tumor tissue.

    • Expression of Sarcomeric Alpha Actinin in human tissue.

    • Expression of Sarcomeric Alpha Actinin in mouse & rat tissue.