PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IHC-P | 1:250 | Ms |
| IF | 1:100 | Ms |
CD4 is a glycoprotein found on the surface of immune cells, primarily known for its role as a coreceptor on T-helper cells, a subset of T lymphocytes. CD4 is used as a marker to distinguish between different T cell subsets, particularly T-helper cells (CD4+ T cells) and T-regulatory cells (also known as Tregs, which can be CD4+CD25+). CD4 acts as a coreceptor with the T cell receptor (TCR) to recognize antigens presented on the surface of antigen-presenting cells (APCs) by MHC class II molecules. This interaction is crucial for the activation of T-helper cells. CD4+ T cells are central to the regulation of immune responses. They help activate B cells, macrophages, and other immune cells, and they produce cytokines that influence the type of immune response (e.g., TH1, TH2, Tfh, Tregs).
IHC shows positive staining in paraffin-embedded mouse cerebral cortex (T cells). Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Expression of CD4 in mouse & rat tissue.
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