PBS, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 5 μl per million cells in 100μl volume | Ms |
Ly-6G protein is a GPI (glycosylphosphatidylinositol)-anchored protein. Ly-6G is primarily expressed on monocytes, neutrophils, and T cells. It plays a crucial role in inhibiting anti-tumor efficacy and T-cell responses. Due to its specific expression on neutrophils, Ly-6G has been widely used as a surface marker in flow cytometry, cell sorting, and immune phenotyping experiments. Unique expression of Ly-6G in bone marrow indicates its involvement in hematopoietic microenvironment, potentially regulating the development or function of immune cells within the bone marrow niche.
Flow cytometric analysis of Mouse Ly-6G/Ly-6C(Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b antibody and either APC-Cy7 Rat IgG2b, κ Isotype Control (Left panel) or SDT APC-Cy7 Rat Anti- Mouse Ly-6G/Ly-6C(Gr-1) antibody (Right panel) at 0.1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.