应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 5 μl per million cells in 100μl volume | Ms |
Mouse I-A/I-E refers to the murine major histocompatibility complex class II (MHC class II) molecules, which play a central role in the immune system by presenting antigens to T cells. The I-A and I-E molecules are expressed on the surface of antigen-presenting cells (APCs), such as B cells, macrophages, and dendritic cells, and are responsible for displaying peptides derived from extracellular proteins to CD4+ T cells. This interaction is crucial for initiating the adaptive immune response. Antibodies against mouse I-A/I-E can be used in various scientific applications, including flow cytometry, immunohistochemistry, and immunoprecipitation. These antibodies can target MHC class II molecules in different species, including mice, and are essential tools for research in immunology and cell biology.
Flow cytometric analysis of Mouse I-A/I-E expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody and either PE-Cy7 Rat IgG2b, κ Isotype Control (Left panel) or SDT PE-Cy7 Rat Anti- Mouse I-A/I-E antibody (Right panel) at 0.01 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.