PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Ms |
| IF | 1:500 | Ms |
This antibody has been tested to ensure minimal cross-reaction with human, Bovine, Horse serum proteins.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling NA/LE Mouse anti-human CD28 mAb
(S0B0010) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) at 1/2000 was used as the secondary antibody. Negative control: THP-1
IF shows positive staining in paraffin-embedded human esophagus. Anti-CD44V6 antibody (S0B0614) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti- CD44V6 antibody(S0B0614) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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