PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Rb |
| IF | 1:500 | Rb |
IF shows positive staining in paraffin-embedded human liver cancer. Anti-Claudin 1 antibody (S0B2354) was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin 1 antibody (S0B2354) was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human cervical cancer. Anti- -Claudin 1 antibody (S0B2354) was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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