12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:2000 | Rt |
| IF | 1:2000 | Rt |
ICC shows positive staining in HeLa cells. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/2000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded mouse testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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