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Mouse IL-2 OneStep ELISA Kit

Mouse IL-2 OneStep ELISA Kit

货号: S0C3020
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    IL-2
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      板内变异系数(Intra-assay):3.5%;
      板间变异系数(Inter-assay):5.1%
    • Sample type

      Cell culture supernatant, Serum,Plasm
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.133pg/ml

    • Range

      1.95pg/mL - 125pg/mL
    • Recovery

      Cell culture supernatant 85%
      Serum 108%
      Plasm 90%
    • Assay time

      60 minutes
    • Species reactivity

      Mouse
    • CROSS REACTIVITY

      /
    • INTERFERENCE

      /
    • NIBSC convert

      /
背景介绍
  • Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL­2 shares 56% and 73% aa sequence identity with human and rat IL­2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL­2 may be a key cytokine in the natural suppression of autoimmunity.

  • ELISA

    • Standard curve
      Example of Mouse IL-6 standard curve in Assay Diluent #6.

    • Linearity
      The concentrations of mouse IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows:  1 unimmunized mouse serum (S#1) and 7 individual immunized mouse serum were measured.

    • Leading Competitor comparison

    • Protocol Diagram