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Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb (S-985-212)

Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb (S-985-212)

货号: S0B1103
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-p90 RSK (T359/S363)

  • 分子别名

    Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); RPS6KA1; MAPKAPK1A; RSK1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q15418

  • 克隆号

    S-985-212

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HepG2, HeLa

  • 预测反应种属
    (反应种属缩写表)

    Ck, Xe

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu,Ms,Rt
背景介绍

  • p90 RSK, also known as RSK1, is a member of the 90 kDa ribosomal S6 kinase family, which includes RSK1, RSK2, and RSK3. These are serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. RSK1 is activated by MAPK in vitro and in vivo via phosphorylation. Active RSKs play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB, which are key regulators of gene expression. The phosphorylation of RSK1 at T359/S363 is part of the signaling cascade initiated by growth factors and hormones, which leads to cellular proliferation and survival.

  • 免疫印迹

    • WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
      Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HepG2 whole cell lysate 20 µg
      Lane 2: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
      Lane 3: untreated HeLa whole cell lysate 20 µg
      Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 83 kDa
      Observed MW: 90 kDa

    • WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
      Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
      Lane 2: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
      Lane 3: HepG2 treated with 100 ng/ml EGF for 20 minutes, then treated with phosphatase whole cell lysate 20 µg
      Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes, then treated with phosphatase whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 83 kDa
      Observed MW: 90 kDa

  • 斑点杂交

    • Dot blot result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
      Lane 1: p90 RSK (T359/S363) phospho peptide
      Lane 2: p90 RSK (S363) phospho peptide
      Lane 3: p90 RSK (T359) phospho peptide
      Lane 4: p90 RSK unmodified peptide
      Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution