12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Syntenin is a multifunctional intracellular adaptor protein that plays a crucial role in various signaling pathways and cellular functions. It is composed of two adjacent tandem PDZ (PSD-95/Discs large/Zonula occludens-1) domains, which recognize multiple peptide motifs with low to moderate affinity. Syntenin interacts with a plethora of proteins, regulating the architecture of the cell membrane. Increases in Syntenin levels can induce the metastasis of tumor cells, neurite protrusion in neuronal cells, and exosome biogenesis in various cell types. It is also involved in the subcellular trafficking of binding partners during endocytic and exocytic events. Additionally, syntenin interacts with the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2), which influences its localization and function at the plasma membrane. Furthermore, syntenin is involved in the regulation of exosome biogenesis, which is a key process in intercellular communication, including neuron-neuron, neuron-glia, and glia-glia crosstalk, as well as in tumor metastasis, cell proliferation, and angiogenesis. Syntenin interacts with proteins involved in exosome biogenesis, indicating its role in intracellular membrane rearrangement and cargo loading into exosomes.
WB result of Syntenin Recombinant Rabbit mAb
Primary antibody: Syntenin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 32 kDa
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti- Syntenin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of Syntenin in tumor tissue.
Expression of Syntenin in human tissue.
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