PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms |
| IF | 1:500 | Hu |
ENPP3 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 3) is a type II transmembrane protein belonging to the membrane-bound ectonucleotide pyrophosphatase/phosphodiesterase family (ENPPs/ENPPs). ENPP3 is involved in nucleotide signaling, particularly in regulating allergic responses in basal cells and mast cells, and serves as a potential biomarker for allergen sensitivity. Under pathological conditions, ENPP3 is overexpressed in various cancers, including renal cell carcinoma, hepatocellular carcinoma, colorectal cancer, and cholangiocarcinoma, making it a potential tumor marker and therapeutic target. ENPP3 is recognized as a major cGAMP hydrolase, which is a second messenger synthesized after the detection of cytosolic double-stranded DNA and is transmitted intercellularly to promote downstream immune signaling. Like ENPP1, ENPP3 inhibits anti-tumor immune responses by hydrolyzing cGAMP.
WB result of ENPP3/CD203c Recombinant Rabbit mAb
Primary antibody: ENPP3/CD203c Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: KU-812 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100 kDa
Observed MW: 150 kDa
WB result of ENPP3/CD203c Recombinant Rabbit mAb
Primary antibody: ENPP3/CD203c Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100 kDa
Observed MW: 140 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti- ENPP3/CD203c antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Expression of ENPP3/CD203c in tumor tissue.
Expression of ENPP3/CD203c in human tissue.
Expression of ENPP3/CD203c in mouse & rat tissue.
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