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NaK-ATPase Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-1373-39)

NaK-ATPase Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-1373-39)

货号: S0B1668
价格: 1056
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    NaK-ATPase
  • 分子别名

    Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Melanosome, Cell membrane
  • Accession

    P05023
  • 克隆号

    S-1373-39
  • 抗体类型

    Recombinant mAb
  • 应用

    IF
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 标记

    Alexa Fluor® 488
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
IF 1:200 Hu
背景介绍
  • NaK-ATPase, also known as the sodium-potassium adenosine triphosphatase (Na+/K+-ATPase) or simply the sodium pump, is a crucial transmembrane protein found in the plasma membrane of animal cells. Utilizing the energy released from ATP hydrolysis, Na+/K+-ATPase actively transports Na+ ions out of the cell against their concentration gradient while simultaneously transporting K+ ions into the cell. This ion transport activity is essential for maintaining the electrochemical gradients across the cell membrane, contributing to the stability of the intracellular and extracellular environments. In addition to its role as an ion pump, Na+/K+-ATPase has signaling functions. It can serve as a receptor for certain hormones and drugs, participating in intracellular signaling cascades.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NaK-ATPase antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- NaK-ATPase antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.