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Mouse anti-Human CD28 Antibody (S-R547)

Mouse anti-Human CD28 Antibody (S-R547)

货号: S0B1082
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD28
  • 分子别名

    T-cell-specific surface glycoprotein CD28; TP44
  • 细胞定位

    Cell membrane
  • Accession

    P10747
  • 克隆号

    S-R547
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 阳性样本

    Human PBMC
  • 纯化方式

    Protein G
  • 浓度

    2 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度
应用 稀释度 推荐种属
FCM 1:2000 Hu
背景介绍
  • CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.

  • 流式分析

    • Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Human CD28 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Then cells were stained with CD19 - Brilliant Violet 421™ separately. Gated on total viable cells.