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Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360)

Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360)

货号: S0B1094
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Stat3 (Tyr705)

  • 分子别名

    Signal transducer and activator of transcription 3; Acute-phase response factor; APRF

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P40763

  • 克隆号

    S-515-360

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Jurkat treated with IFN-α, HeLa treated with IFN-α

  • 预测反应种属
    (反应种属缩写表)

    Pg, Cw

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu
IP 1:50 Hu
背景介绍

  • Phospho-Stat3 (Tyr705) refers to the phosphorylated form of Signal Transducer and Activator of Transcription 3 (STAT3) at the tyrosine 705 residue. Phosphorylation of STAT3 at Tyr705 is a critical step in the activation of the STAT3 signaling pathway. This phosphorylation event, often induced by cytokines, growth factors, and oncogenes, leads to the formation of STAT3 dimers, which then translocate to the nucleus to initiate gene transcription. STAT3 is involved in various cellular processes, including cell growth, survival, and immune responses. Phospho-Stat3 (Tyr705) is essential for mediating cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. In the context of immunity, STAT3 plays a crucial role in regulating the differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg), which is controlled by the phosphorylation at Tyr705. Abnormal activation of STAT3, including constitutive phosphorylation at Tyr705, has been observed in various cancers and is associated with tumor growth, survival, and angiogenesis. In some cases, STAT3 activation is driven by autocrine secretion of interleukin-6 (IL-6), leading to continuous activation of the Jak/Stat pathway.

  • 免疫印迹

    • WB result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
      Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: untreated Jurkat whole cell lysate 20 µg
      Lane 2: Jurkat treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
      Lane 3: untreated HeLa whole cell lysate 20 µg
      Lane 4: HeLa treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 88 kDa
      Observed MW: 88 kDa

  • 免疫沉淀

    • Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate 40 µg (Input)
      Lane 2: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb IP in Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate
      Predicted MW: 88 kDa
      Observed MW: 88 kDa
      Exposure time: 90 s

  • 斑点杂交

    • Dot blot result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
      Lane1: Stat3 (Tyr705) phospho peptide
      Lane2: Stat3 unmodified peptide
      Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution