PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms |
TIM-3 (T cell immunoglobulin and mucin domain-containing protein 3), also known as HAVCR2, is a crucial immune checkpoint molecule that is extensively expressed on various immune cells, including Th1, Th17, Treg, dendritic cells, monocytes, macrophages, and NK cells. The role of TIM-3 in the tumor immune microenvironment is particularly significant, as it is highly expressed on tumor-infiltrating CD8+ T cells and regulatory T cells (Tregs), and is co-expressed with PD-1, participating in the regulation of tumor immune evasion and T cell exhaustion. TIM-3 exerts its immune suppressive function by binding to its ligands, which include galectin-9 (Gal-9), phosphatidylserine (PtdSer), carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), and high mobility group box 1 (HMGB1). These interactions lead to Th1 cell apoptosis, T cell exhaustion, and immune suppression, thereby facilitating tumor cells to evade immune surveillance. In cancer treatment strategies, TIM-3 has emerged as a promising target. Furthermore, the expression level of TIM-3 correlates with poor prognosis in certain types of cancer, making it a potential prognostic biomarker.
WB result of Invivo Anti-Mouse CD366 (TIM-3) Recombinant mAb
Primary antibody: Invivo Anti-Mouse CD366 (TIM-3) Recombinant mAb at 1/1000 dilution
Lane 1: TIM-3/HAVCR2 His Tag Protein, Mouse 2 μg
Lane 2: TIM-3/HAVCR2 His Tag Protein, Human 2 μg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33~55 kDa
Observed MW: 33~50 kDa
This blot was developed with high sensitivity substrate
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