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Mouse Ig isotype OneStep ELISA Kit

Mouse Ig isotype OneStep ELISA Kit

货号: S0C3015
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    Ig
  • 免疫原

    Recombinant Protein
  • 反应种属 ?

    Ms
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: NA;

      Inter-assay: NA

    • Sample type

      Cell culture supernatant
    • Assay type

      Sandwich (qualitatively)
    • Assay time

      15 minutes
    • Species reactivity

      Mouse

    • CROSS REACTIVITY

      /
    • INTERFERENCE

      /
    • NIBSC convert

      /
背景介绍
  • In immunology, antibodies (immunoglobulins (Ig)) are classified into several types called isotypes or classes. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mice, same as humans. Each isotype has a different heavy chain. Isotypes are also called classes. Another name for antibodies is immunoglobulin, hence the suffix of 'Ig' to designate antibody classes and subclasses. Naive B cells produce IgM and IgD. During B cell maturation, through isotypic switching, a mature B cell will produce one of IgG, or IgA, or IgE isotypes and subclasses. Different isotypes have different half-lives in vivo, ranging from 12 hours to 8 days.

  • ELISA

    • Standard curve

    • Protocol Diagram