PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000-1:10000 | Species Independent |
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.
WB result of FLAG Tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: 293T transfected with Histone H3-Flag-V5 whole cell lysate 20 µg
Primary antibody:
Lane 1: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
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