PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:100 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
| ICFCM | 1:50 |
SMARCA2, also known as BRM (Brahma-related gene on chromosome X), is a subfamily member of the SWI/SNF family of proteins. It plays a crucial role in chromatin remodeling, which involves the adjustment of chromatin structure to facilitate or hinder the access of the transcriptional machinery to DNA. This process is essential for various cellular functions, including gene expression, DNA repair, and replication. As a part of the SWI/SNF complex, SMARCA2 uses the energy from ATP hydrolysis to reposition and evict nucleosomes, thereby altering chromatin structure. This function is vital for transcriptional regulation and maintaining genomic integrity. Mutations or alterations in the expression of SMARCA2 have been implicated in various human diseases, particularly in the context of cancer. It has been found that SMARCA2 mutations can lead to different neurodevelopmental disorders, including Nicolaides-Baraitser syndrome (NCBRS) and Lennox-Gastaut syndrome (LGS). These conditions are often characterized by developmental delays, intellectual disabilities, and epilepsy.
WB result of SMARCA2/BRM Recombinant Rabbit mAb
Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 220 kDa
WB result of SMARCA2/BRM Recombinant Rabbit mAb
Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 220 kDa
WB result of SMARCA2/BRM Recombinant Rabbit mAb
Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 230 kDa
This blot was developed with high sensitivity substrate
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SMARCA2/BRM antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- SMARCA2/BRM antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- SMARCA2/BRM antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells (top panel) and negative staining in NCCIT cells (below panel). Anti-SMARCA2/BRM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of SMARCA2/BRM in tumor tissue
Expression of SMARCA2/BRM in human tissue
Expression of SMARCA2/BRM in mouse & rat tissue
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