PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Ms |
| ICC | 1:500 | Ms |
Phospho-PAK2 (Ser20) refers to the p21-activated kinase 2 (PAK2) protein that has been phosphorylated at the serine residue number 20. PAK2 is a serine/threonine kinase involved in various signaling pathways, including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. When active, CDC42 and RAC1 bind to PAK2, causing a conformational change and subsequent autophosphorylation on several serine and/or threonine residues, including Ser20. Phosphorylation at Ser20 is significant because it can affect the activation state of PAK2 and its ability to interact with other proteins. For instance, phosphorylation of PAK2 at Ser20 has been implicated in the regulation of the actin cytoskeleton and T cell receptor (TCR) signaling, which are crucial for normal thymocyte development and maturation. Additionally, the phosphorylation status of PAK2 can serve as a marker for cellular processes and can be detected using specific antibodies designed to recognize the phosphorylated form of the protein.
WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Lane 3: untreated Jurkat whole cell lysate 20 µg
Lane 4: Jurkat strave overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 62 kDa
WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 62 kDa
WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 62 kDa
Phospho-PAK2 (Ser20) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-PAK2 (Ser20) in 0.4 mg NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-PAK2 (Ser20) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-PAK2 (Ser20) Rabbit mAb IP in NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate
Predicted MW: 58 kDa
Observed MW: 62 kDa
ICC analysis of NIH/3T3 cells starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes (top panel) and NIH/3T3 cells starved for 3 hours then untreated with Calyculin A (100nM) for 30 minutes (below panel). Anti- Phospho-PAK2 (Ser20) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
您现在的位置：