12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
| FCM | 1:2000 | Hu |
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
WB result of Mouse anti-Human CD90 Antibody
Primary antibody: Mouse anti-Human CD90 Antibody at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: HuT 78 whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 23~37 kDa
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling Human CD90 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: K562
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD90 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of CD90 in tumor tissue
Expression of CD90 in human tissue
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