12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms |
| IHC-P | 1:1000 | Ms |
CD3ε, also known as the epsilon chain of the CD3 complex, is a crucial component of the T cell receptor (TCR) complex, which plays a central role in T cell activation and antigen recognition. CD3ε is particularly important in the context of T cell-mediated immune responses. It has been identified as a key target in the development of immunotherapies for cancer. Recent research has also highlighted the role of CD3ε in CAR-T cell therapy. By incorporating the CD3ε cytoplasmic domain into a chimeric antigen receptor (CAR), the antitumor activity of CAR-T cells can be improved.
WB result of CD3ε Recombinant Rabbit mAb
Primary antibody: CD3ε Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: EL4.IL2 whole cell lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of CD3ε in mouse & rat tissue
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