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His tag OneStep ELISA Kit

His tag OneStep ELISA Kit

货号: S0C3016
价格: 2800
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    His tag
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Species Independent
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Sample type

      N-terminal/C-terminal/internal His-tagged proteins.
      Mammalian and bacteria cell lysates or cell supernatant.
    • Assay type

      Competitive (quantitative)
    • Sensitivity

      4 ng/ml
    • Range

      4 ng/ml -1000 ng/ml
    • Assay time

      60 minutes
背景介绍
  • A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins. 

  • ELISA

    • Standard curve
      Example of His tag protein (molecular weight of 20 kDa) standard curve in Assay Diluent #1.

    • Standard curve
      Example of His tag protein (molecular weight of 20 kDa) standard curve in Assay Diluent #1.

    • Reagent Compatibility

    • Protocol Diagram