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Rabbit Anti-Myc tag agarose beads

Rabbit Anti-Myc tag agarose beads

货号: S0B1642
价格: 800
规格: 125μl(5reactions)
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    Co-IP, Protein purification, IP
  • 反应种属 ?

    Species Independent
  • 纯化方式

    Protein A
  • 标记

    Agarose beads
  • 性状

    Liquid
  • 缓冲体系

    50% slurry in PBS pH7.4, 0.05% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
背景介绍
  • The c-Myc is a polypeptide protein tag derived from the c-myc gene product, which can be added to the protein by recombinant DNA technology. It contains 10 amino acids (EQKLISEEDL) with a molecular weight of 1.2 KDa and can be fused to the N-terminus or C-terminus of the target protein for purification and detection. The Myc tag is widely used in various expression systems, including bacteria, yeast, insects and mammalian cells, to detect the expression of fusion proteins. It can be used for affinity chromatography and purified by coupling the Myc-tagged antibody to divinylsulfone-activated agarose. 

  • 电泳JSON

    • Rabbit Anti-Myc tag agarose Beads were used to immunoprecipitate 2 x Myc tag-mouse IgE-His tag from Expi293F system. 
      During elution with Pepide elution buffer, the enriched Myc fusion protein are released from the agarose beads. 
      SDS-PAGE analysis was performed.
      Lane 1: Input
      Lane 2: Flow-through
      Lane 3: 2 x Myc tag-mouse IgE-His tag protein.

    • Rabbit Anti-Myc tag agarose Beads were used to immunoprecipitate 2 x Myc tag-mouse IgE-His tag from Expi293F system. 
      During elution with Pepide elution buffer, the enriched Myc fusion protein are released from the agarose beads. 
      SDS-PAGE analysis was performed.
      Lane 1: Input
      Lane 2: Flow-through
      Lane 3: 2xMyc tag-mouse IgE-His tag protein.