PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
Phospho-NF-κB p65 (Ser536) refers to the phosphorylation of the p65 subunit of the NF-κB protein complex at the serine 536 residue. This post-translational modification is crucial for regulating the transcriptional activity, nuclear localization, and protein stability of NF-κB, which is a master regulator of inflammation and cell survival. Phosphorylation at Ser536 has been shown to negatively regulate NF-κB p65 activity. In a study published in Sci Signal, it was demonstrated that mice expressing a mutant p65 with an alanine-to-serine substitution at position 534 (the murine homolog of human Ser536) exhibited increased expression of NF-κB-dependent genes upon inflammatory stimulation, leading to enhanced inflammation and increased mortality. The phosphorylation status of NF-κB p65 at Ser536 is also associated with the prognosis of certain cancers. For instance, in colorectal cancer, increased expression of phospho-Ser536-p65 in the cytoplasm of primary tumors correlates with worse patient survival, independent of other clinical factors, making it an independent prognostic factor. Moreover, the phosphorylation of NF-κB p65 at Ser536 has been implicated in the pathogenesis of liver cancer.
WB result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDa
WB result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDa
Dot blot result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Lane 1: NF-κB p65 (Ser536) phospho peptide
Lane 2: NF-κB p65 unmodified peptide
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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