PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:5000 | Ms |
The CD8 antigen is a cell surface glycoprotein primarily found on most cytotoxic T lymphocytes (CTLs). It acts as a co-receptor, along with the T-cell receptor (TCR), to recognize antigens presented by antigen-presenting cells (APCs) in the context of class I MHC molecules. The functional co-receptor can be either a homodimer composed of two alpha chains or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology with immunoglobulin variable light chains. In research, CD8B plays a central role in the development and function of T cells. For example, it is involved in the positive and negative selection processes of T cells in the thymus, as well as the regulation of mature single-positive CD8+ T cells being released into the circulation. Moreover, the expression of the CD8B gene is finely regulated, including the regulation of CD4 and CD8A gene expression during thymic differentiation.
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse CD8β (Lyt 3.2) antibody at 1/5000 (0.1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8α - Alexa Fluor® 647 separately. Gated on total viable cells.
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse CD8β (Lyt 3.2) antibody at 1/5000 (0.1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ separately. Gated on total viable cells.
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