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Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (S-R440)

Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (S-R440)

货号: S0B1632
价格: 650
规格: 20T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD16
  • 分子别名

    Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; FCGR3A
  • 细胞定位

    Cell membrane
  • Accession

    P08637
  • 克隆号

    S-R440
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 阳性样本

    human PBMC
  • 纯化方式

    Protein G
  • 浓度

    0.2mg/ml
  • 标记

    Alexa Fluor® 488
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度 推荐种属
FCM 5 μl per million cells in 100μl volume Hu
背景介绍
  • CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. These receptors bind to the Fc portion of IgG antibodies, which then activates antibody-dependent cell-mediated cytotoxicity (ADCC) in human NK cells. CD16 is required for ADCC processes carried out by human monocytes. In humans, monocytes expressing CD16 have a variety of ADCC capabilities in the presence of specific antibodies, and can kill primary leukemic cells, cancer cell lines, and cells infected with hepatitis B virus. In addition, CD16 is able to mediate the direct killing of some virally infected and cancer cells without antibodies.

  • 流式分析

    • Flow cytometric analysis of Human CD16 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Phycoerythrin Mouse Anti-Human CD56 antibody and either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.